Shalini Vidyadharan Project Associate
Email
[email protected]
Phone
8078178577
Location
Punnaritta Madom, Vazhar mangalam P.O.,Chengannur, Kerala, India
Education
12/2020 – 02/2023 | Hyderabad
  • •Fellowship by Department of Biotechnology, Govt. of India
  • •CGPA: 8.26
  • •Dissertation title: Study of Long-term Memory Using Courtship in Drosophila

    • Advisor/PI: Dr. Amitabha Majumdar

    06/2017 – 08/2020 | Kollam
  • •Merit-cum-Means Scholarship Scheme, Govt. of India
  • •CGPA: 9.02
    • Research experience

    Project Associate

    CSIR-Centre for Cellular and Molecular Biology(CCMB)
    12/2023 – Present | Hyderabad

    Advisor/PI: Dr. Anant Bahadur Patel

    Investigating brain activity and neurotransmitter flux changes in various mice models:

  • •Type-2 Diabetes: Quantified cell-type specific neurotransmitter flux in lepr(db/​db) mice using ¹H-[¹³C]-NMR spectroscopy, revealing region-specific excitatory (34–40%) and inhibitory (47–90%) hyperactivity with preserved astrocytic metabolism. Manuscript under review.
  • •Every-Other-Day Fasting: Identified hypothalamic-selective neuroprotection across aging cohorts using ¹H-[¹³C]-NMR; validated AMPK downregulation and led TMTpro-16plex proteomics sample preparation for pathway analysis. Manuscript in preparation.
  • •Chronic Ketamine (Collaborative): Characterised molecular effects of sub-anesthetic ketamine using RNA sequencing with qRT-PCR validation. Manuscript in preparation.

    • Master's Dissertation Research

    National Centre for Cell Science (NCCS), Pune
    06/2022 – 11/2022 | Pune

    Advisor/PI: Dr. Amitabha Majumdar

    Project Title: Study of Long-Term Memory Using Courtship in Drosophila

  • •Demonstrated that Flotilin is required for long-term memory retention in Drosophila using courtship conditioning assays in KO models.
    • Laboratory Experience
    Mice Handling: Behavioral tests (FST, OFT, EPM, Y-maze, Morris Water maze, NORT ). | Tail vein infusion, perfusion techniques, region-specific brain microdissection, and Surgeries (stereotaxic).
    Fly handling and maintenance: Sexing of flies, Virgin collection, Courtship assay, Adult brain dissection, Larval brain dissection, NMJ dissection
    Molecular Biology: DNA/RNA and Plasmid isolation, qPCR, AGE, PAGE, Western blotting, Immunohistochemistry(IHC) and Protein purification using coloumn chromatography, Fluorescence-Activated Cell Sorting(FACS)
    Quantitative Proteomics: Global and phosphoproteomics analysis of mouse brain tissue with iTRAQ/TMT labeling | Protein isolation, quantification (BCA assay), peptide generation using in-solution method (FASP, Desalting) | Basic pH Reverse Phase HPLC-based Fractionation | Tandem mass spectrometry - LC-MS/MS analysis
    NMR Spectroscopy: ¹H-[¹³C]-NMR spectroscopy