Shalini Vidyadharan Project Associate
Email
[email protected]
Phone
8078178577
Location
Punnaritta Madom, Vazhar mangalam P.O.,Chengannur, Kerala, India
Education
12/2020 – 12/2022Hyderabad
  • •CGPA: 8.26
  • •Dissertation title: Study of Long-term Memory Using Courtship in Drosophila

    • Advisor/PI: Dr. Amitabha Majumdar

    06/2017 – 08/2020Kollam
  • •CGPA: 9.02
    • Industrial Experience

    Associate Content Editor

    Clarivate Analytics
  • •Annotated molecular interactions from high-impact journals for database curation, with a focus on protein-protein, DNA/RNA-protein, and DNA/RNA-DNA/RNA interactions.
    • 06/2023 – 12/2023
    Research Experience

    Project Associate I

    CSIR-Centre for Cellular and Molecular Biology(CCMB)

    Advisor/PI: Dr. Anant Bahadur Patel

    12/2023 – presentHyderabad, India
  • •Designed and conducted pilot studies on rodent models (Db/Db and dietary models) to analyse behavioural changes, brain (neuronal and astroglial) activity, and energy metabolism patterns.
  • •Mastered advanced ¹H-[¹³C]-NMR spectroscopy technique to elucidate metabolic perturbations in critical biochemical pathways, including the TCA cycle and establish correlations with brain activity patterns.
  • •Optimised protocol for global and phosphoproteomics using iTRAQ / TMT label(LC-MS/MS analysis).
  • •Ongoing:
  • •Performing qPCR experiments to validate RNA sequencing findings for manuscript titled "Assessing the Effect of Chronic Ketamine on Molecular, Behavioral and Metabolic Changes in the Brain"
  • •Manuscript preparation of understanding the impact of Type-2 Diabetes on cortical, hypothalamic neuronal activity and their energy metabolism.

    • Master's Dissertation Research

    National Centre for Cell Science (NCCS), Pune

    Project Title: Study of Long-Term Memory Using Courtship in Drosophila

    06/2022 – 11/2022

    Advisor/PI: Dr. Amitabha Majumdar

  • •Investigated the interaction between Flotilin and Orb2 proteins in memory formation through courtship assays in knockout Drosophila models.
  • •Conducted protein purification using metal-binding affinity chromatography to purify Tau protein and then attempted to phase separate it using fluorescence microscopy.
    • Laboratory Experience
    Mice Handling: Behavioral tests (eg., FST, OFT ). | Tail vein infusion, perfusion techniques, region-specific brain dissection, and Surgeries (stereotaxic).
    Fly handling and maintanance: Sexing of flies, Virgin collection, Courtship assay, Adult brain dissection, Larval brain dissection, NMJ dissection
    Molecular Biology: DNA/RNA and Plasmid isolation, qPCR, AGE, PAGE, Western blotting, Immunohistochemistry(IHC) and Protein purification using coloumn chromatography, Fluorescence-Activated Cell Sorting(FACS)
    Quantitative Proteomics: Global and phosphoproteomics analysis of mouse brain tissue with iTRAQ/TMT labeling | Protein isolation, quantification (BCA assay), peptide generation using in-solution method (FASP, Desalting) | Basic pH Reverse Phase HPLC-based Fractionation | Tandem mass spectrometry - LC-MS/MS analysis | Analysis using proteome discoverer and IPA(integrative pathway analysis).
    NMR Spectroscopy: ¹H-[¹³C]-NMR spectroscopy
    Software and Data Analysis: GraphPad Prism | Noldus EthoVision XT | Proteome discoverer | MATLAB
    Conference Presentation

    AIM-AT, IAN-2024

    Shalini, & Patel, A. B., “Assessing the Impact of Every-Other-Day Fasting on Brain Metabolism in a Mouse Model of Aging.” Poster presentation delivered at the International Conference on Advances In Mechanisms and Approaches to Neuro-Therapeutics (AIM-AT) & XLII Annual Meeting of the Indian Academy of Neurosciences 2024 (IAN-2024), National Institute of Mental Health and Neurosciences, Bengaluru, India; November 12-14, 2024.

    11th Annual Scientific Meeting of the International Society for Magnetic Resonance in Medicine (ISMRM) Indian Chapter

    Attended this meeting (2025, February 27–March 1), Theme: MR Updates on Acquisition, Reconstruction, Analysis, AI, and Beyond, Indian Institute of Technology (IIT Hyderabad). Won 1st prize in MRI quiz

    Teaching/Outreach Experience

    Skill Development Program in Metabolomics and NMR spectroscopy

    Centre for Cellular and Molecular Biology (CCMB) Volunteer Instructor
    January 27 - 31 , 2025
    HONORS & AWARDS
  • •Silver Rank in BSc Microbiology, Amrita School of Biotechnology
  • •GAT-B qualified 2020 AIR 87
  • •CSIR-NET 2023(LS) AIR 43
    • References
    Dr. Anant Bahadur Patel, Chief Scientist, Centre for Cellular and Molecular Biology (CCMB), Hyderabad
    Dr. A. Venkateshwari, Professor, Institute of Genetics and Hospital for Genetic Diseases, Hyderabad