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SHALINI
Email
[email protected]
Phone
8078178577
Location
Punnaritta Madom, Vazhar mangalam P.O.,Chengannur, Kerala, India
Education
12/2020 – 12/2022Hyderabad
  • •CGPA: 8.26
  • •Dissertation title: Study of Long-term Memory Using Courtship in Drosophila

    • Advisor/PI: Dr. Amitabha Majumdar

    06/2017 – 08/2020Kollam
  • •CGPA: 9.02
  • •Dissertation title: Determination of SPF and Antioxidant properties of extracts from algae

    • Advisor/PI: Dr. Jayshree G

    Research Experience

    Project Associate I

    Centre for Cellular and Molecular Biology(CCMB)

    Advisor/PI: Dr. Anant Bahadur Patel

    12/2023 – presentHyderabad, India
  • •Led and contributed to pilot projects on rodent models (Db/Db and dietary models) to analyze their behavior, brain (neuronal and astroglial) activity, and their energy metabolism.
  • •Gained expertise in ¹H-[¹³C]-NMR spectroscopy to study metabolic changes in various biochemical pathways like the TCA cycle and correlate with brain activity.
  • •Optimised protocol for global and phosphoproteomics using iTRAQ / TMT label.
  • •Ongoing:
  • •Performing molecular studies like western blot, Immunohistochemistry, and qPCR to validate omics data.
  • •Manuscript preparation of understanding the impact of Type-2 Diabetes on cortical, hypothalamic neuronal activity and their energy metabolism.

    • Master's Dissertation Research

    National Centre for Cell Science (NCCS), Pune

    Project Title: Study of Long-Term Memory Using Courtship in Drosophila

    06/2022 – 11/2022

    Advisor/PI: Dr. Amitabha Majumdar

  • •Investigated the interaction between Flotilin and Orb2 proteins in memory formation through courtship assays in knockout Drosophila models.
  • •Conducted protein purification using metal-binding affinity chromatography to purify Tau protein and then attempted to phase separate it using fluorescence microscopy.
    • Industrial Experience

    Associate Content Editor

    Clarivate Analytics
  • •Annotated molecular interactions from high-impact journals for database curation, with a focus on protein-protein, DNA/RNA-protein, and DNA/RNA-DNA/RNA interactions.
    • 06/2023 – 12/2023
    Laboratory Experience
    Mice Handling: Behavioral tests (eg., FST, OFT ). | Tail vein infusion, perfusion techniques, region-specific brain dissection, and Surgeries (stereotaxic).
    Fly handling and maintanance: Sexing of flies, Virgin collection, Courtship assay, Adult brain dissection, Larval brain dissection, NMJ dissection
    Clinical Field: Experience interacting with patients having various genetic disorders
    Molecular Biology: DNA/RNA and Plasmid isolation, qPCR, AGE, PAGE, Western blotting, Immunohistochemistry(IHC) and Protein purification using coloumn chromatography
    Quantitative Proteomics: Global and phosphoproteomics analysis of mouse brain tissue with iTRAQ/TMT labeling
    NMR Spectroscopy: ¹H-[¹³C]-NMR spectroscopy
    Microscopic techniques: Bright field and fluorescence microscopy
    Microbiology: Bacterial transformation (CaCl2 method), Spread plate, Pour plating, Staining techniques
    Software and Data Analysis: GraphPad Prism, Noldus EthoVision XT, MATLAB
    Internships and Training

    Centre for DNA Fingerprinting and Diagnostics

    15 days Internship Training
  • •Completed a 15-day internship, gaining exposure to advanced diagnostic techniques and laboratory procedures.
    • 2022Hyderabad
  • •Observed key departments, including the Diagnostic Lab, Sophisticated Equipment Lab, and National Genomics Centre.

    • National Institute of Animal Biotechnology

    3 day training program
  • •Participated in a 3-day training program focused on animal handling and developmental genetics.
    • 2021Hyderabad
  • •Performed experiments on mice models, applying genetic manipulation techniques and enhancing lab-based skills.

    • Mini project: Association of ABCC8 gene polymorphism in Type-I diabetic children

    Institute of Genetics and Hospital for Genetic diseases

    Guide: Dr. G. Shilpa Reddy

    2022
  • •The study focused on finding an association between Abcc8 gene variants and type-I diabetic children by performing RFLP.
    • Conference Presentation

    AIM-AT, IAN-2024

    Shalini, & Patel, A. B., “Assessing the Impact of Every-Other-Day Fasting on Brain Metabolism in a Mouse Model of Aging.” Poster presentation delivered at the International Conference on Advances In Mechanisms and Approaches to Neuro-Therapeutics (AIM-AT) & XLII Annual Meeting of the Indian Academy of Neurosciences 2024 (IAN-2024), National Institute of Mental Health and Neurosciences, Bengaluru, India; November 12-14, 2024.

    11th Annual Scientific Meeting of the International Society for Magnetic Resonance in Medicine (ISMRM) Indian Chapter

    Attended this meeting (2025, February 27–March 1), Theme: MR Updates on Acquisition, Reconstruction, Analysis, AI, and Beyond, Indian Institute of Technology (IIT Hyderabad). Won 1st prize in MRI quiz

    Teaching/Outreach Experience

    Skill Development Program in Metabolomics and NMR spectroscopy

    Centre for Cellular and Molecular Biology (CCMB) Volunteer Instructor
    January 27 - 31 , 2025
    HONORS & AWARDS
  • •Silver Rank in BSc Microbiology, Amrita School of Biotechnology
  • •GAT-B qualified 2020 AIR 87
  • •CSIR-NET 2023(LS) AIR 43
    • References
    Dr. Anant Bahadur Patel, Chief Scientist, Centre for Cellular and Molecular Biology (CCMB), Hyderabad
    Dr. A. Venkateshwari, Professor, Institute of Genetics and Hospital for Genetic Diseases, Hyderabad