Skilled and accomplished cell and molecular biologist with 15+ years of experience in academic and industry settings. Strong background in molecular cloning, mammalian cell culture. Eager to join and work on development of Cell and Gene therapy product in a reputed academic/ Biotech industry

Project handled: Development and characterization of indigenous CAR T cell therapy product targeting the tumor specific antigens of B cell lymphoma

Technical Skills:

• Multiple (>50) variants of CAR gene plasmids were created using conventional, infusion cloning and site directed mutagenesis based on in silico data.

• Isolation of PBMCs from whole blood, Separation, activation and expansion of T and NK cells

• LVV mediated Transduction and nucleofection of primary T cells and cancerous T cell lines

• Cell based assays using Multi color Flow cytometry, ELISA and live cell imaging for determining anti tumour efficacy of CAR T cell, T cell activation and proliferation

• Cytotoxicity assays for determining anti tumour efficacy of CAR T cells

• Developed a bacterial clone for the production of CD19 scFv and purified using affinity tag

•Designed and excuted CRISPR-Cas9 technique for deletion of TRAC1 gene from T cell genome

• Designed and optimised efficacy testing method for in house formulated antifungal lotion

• Managed team of 5 junior scienitists - Responsible for their recuitment, training and Evaluation

• Demomstrated writing and presentation skills - Presenting data and proposed projects to management

• Writing SOPs, Protocols, Organising data and storage

• Established RT-PCR lab for testing the shrimp samples for viral pathogens such as WSSV, EHP etc.

• Taken a lead role for handling of regulatory inspections/training, third party audits for global food safety standards (HACCP, BAP, BRC, FSSAI, USFDA, etc.)

• Testing of microbial load and pathogens in Shrimp samples, water and food contacting surfaces using microbial culture techniques and biochemical tests

• Testing of Pesticide and antibiotic residues in shrimp samples using ELISA method

• Writing SOPs and Documentation for audits

• Established and developed infrastructure for a diagnostics clinical lab with equipment and technical staff

• Overseeing the day-to-day operations of a clinical laboratory

• Ensuring all equipment in the laboratory setting meets safety standards

• Training and supervising clinical laboratory technicians

Project handled: Spinal muscular atrophy (SMA) is an autosomal recessive neuromuscular disease caused by loss or mutation of the survival motor neuron 1 gene SMN1. Objective of the project is to investigate how SMA-linked mutations of SMN1 lead to structural/functional deficiency of SMN macro molecular complex using Saccharomyces cerevisiae as homologous system

Technical Skills:

• Manipulation and tagging of genes in S.cerevisiae with fluoroscent protein genes and affinity tag (STrEP one) using conventional molecular cloning techniques • Purification of native macromolecular SMN protein complexes using Ultracentrifugation and affinity chromatography

• Structural analysis of native macromolecular SMN protein complexes using Cryo EM

• Study of protein - protein interactions using Fluorescent microscope

• Characterization of purified proteins using SDS PAGE and western Blots

Project handled: Investigating the changes in gene expression within the liver of two genetic variants of rainbow trout fish called high stress responders and low stress responders during exposure to a prolonged period of confinement. The obtained transcriptomic profile data can be used for the establishment of biomarker genes against the stress in Rainbow trout.

Technical skills:

• Nucleic acid extractions from different tissues of fish

• cDNA microarrays

• qPCR

• Microarray data analysis using R Bioconductor and GeneSpring software

Submitted thesis entitled: "Construction of yeast reporter system to evaluate the toxic Volatiles released from Wood"

Grade: Magna cum laude

Grade: First class

Worked as a “B” grade guest faculty at Centre for Biotechnology, Acharya Nagarjuna University, Guntur, India, (Aug.2000 to Feb.2003) taught Genetics, Biochemistry for M.Sc Biotechnology students, Food Biotechnology for M. Tech students

Guided two Bachelor’s students in Practicum entitled “An essential gene for fruiting body initiation in the basidiomycete Coprinopsis cinerea is homologous to bacterial cyclopropane fatty acid synthase genes” at Molecular Wood Biotechnology Lab, Georg-August-Universität Göttingen, Germany.